If kept in a relatively warm portion of the freezer - for instance, the freezer section that is -68℃ most of the time - the temperature is often going to exceed -65℃ whenever the door is open for more than a few minutes, potentially causing the biomolecule genetic material to degrade over time. Some biomolecules (e.g., RNA) have a recommended storage temperature of -80℃ and may degrade if stored at higher temperatures.
The temperature displayed on the front of the freezer was -79℃. A temperature mapping exercise indicated a 15℃ difference between the warmest and coldest regions inside the freezer - a range that stretched from -83.5℃ to -68.7℃. To put this into perspective, let’s use the example of an 8-year-old Revco -80℃ freezer at a major university medical center. Detailed temperature measurements of various sections of your freezer - what’s known as temperature mapping - will identify all the warmest and coldest parts of your lab freezer. Since there are wide temperature differences within a normal -80℃ freezer (usually at least an 8℃ difference between the warmest and coldest areas of the appliance), it is best to place the probe in the warmest part of the freezer, which is typically near the top since warm air rises. Temperature probes for -80℃ laboratory freezers measure the current temperature at the very tip of the probe - so proper placement of the probe tip ensures you measure the temperature where it matters most. The Science Behind Temperature Probe Placement To stay on the right side of this equation, you’ll generally want to place temperature probes at the top of the lab freezer - and here’s why.
With the proper placement, you’ll have a clear, accurate understanding of the temperature inside your freezer without it, you can remain in the dark as to potentially devastating freezer temperature issues. Location matters not just in the sense of where you situate your lab freezer, but also where your temperature probe is placed inside the appliance.
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